The detection rate of intestinal protozoa was compared using the Lugol´s iodine method and trichrome staining method. Lugol´s iodine method used following formalin-ether concentration (MGL), a most widely used protozoa examination. The trichrome staining method was performed after preserving the specimen in fixative solution. During the period from April 1986 to February 1987, a total of 390 samples from patients of Wonju Christian Hospital were collected and examined.
The results are summarized as follows
1 . The detection rate of intestinal protozoa was 4.4% by Lugol´s iodine method compared with 14.2% by trichrome staining method.
2 . Four species of protozoan cysts were observed by Lugol´s iodine method, the detection rate of each intestinal protozoa was 1.8% for Endolimax nana, 1.0% for Entamoeba coli, 0.8% for Entamoeba histolvtica, and 0.5% for Giardia lamblia.
3 . Using the trichrome staining method, four species of protozoan trophozoites´ and cysts were detected, and the detection rate of each intestinal protozoa was 6.5% for Endolimax nana, 3.6% for Entamoeba soli, 3.1% for Entamoeba histolytica, and 1.4% for Giardia lamblia.
4 . The sensitivity was 28%, Lugol´s method, 92% by trichrome staining and false negative 72% and 8% respectively. Negative predictive value was 88% by Lugol´s method and 99% by trichrome staining.
So we concluded that the trichrome staining method was the most reliable method of examination of intestinal protozoa.
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